FastQCFastQC Report
Wed 15 Nov 2017
HVH7LAFXX_n01_vs20_171115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH7LAFXX_n01_vs20_171115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6687726
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4650.015.1462981
GCACACG6200.010.28951611
AGTCACG6450.010.23206328
ACACGTC7050.09.67285713
ACGTCTG7300.09.64279215
CACGTCT7850.09.24740614
CGTCTGA7550.08.44941616
GATCGGA7300.08.4419871
CGTTTCG7001.8189894E-128.17109433
GTCACGT7753.6379788E-127.664145529
CACACGT8251.8189894E-127.465970512
AGCACAC10655.456968E-126.403254510
TCGGAAG13101.8189894E-125.88008363
CTCCAGT13154.1836756E-115.520438724
TCCAGTC11305.335096E-95.450859525
CCAGTCA11406.5901986E-95.403166326
CAGTCAC11559.0167305E-95.33307527
GAACTCC11504.5469278E-85.164658521
ACTCCAG13901.0968506E-95.064313423
Produced by FastQC (version 0.11.2)