FastQCFastQC Report
Wed 15 Nov 2017
HVH7LAFXX_n01_vs1_171115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH7LAFXX_n01_vs1_171115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6449624
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1062071.6467161496546157TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG142450.041.9746611
CACACGT143700.041.65513612
CGTCTGA143750.041.5931316
GATCGGA139800.041.5487561
ACACGTC144100.041.5391913
ACGTCTG144050.041.50650415
CACGTCT144650.041.3343414
AGCACAC146400.040.87251710
GAGCACA146700.040.7889339
CGTATGC126300.040.75766844
TCCAGTC144700.040.69738825
TATCTCG125850.040.59096539
TCGGAAG147900.040.5765763
CCGATGT144200.040.55053733
AGAGCAC148050.040.506158
CACCGAT145600.040.37184531
GTCACCG145550.040.35548429
GAACTCC146800.040.3393621
CAGTCAC146550.040.30528627
CGATGTA145050.040.29743234
Produced by FastQC (version 0.11.2)