FastQCFastQC Report
Wed 15 Nov 2017
HVH7LAFXX_n01_vs19_171115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH7LAFXX_n01_vs19_171115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6838949
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTGG9450.023.27940831
GCACACG11100.020.61171511
AGTCACG11100.020.01714528
TCACGTG11750.019.28426730
ACACGTC11950.018.96096213
GATCGGA12250.018.6853451
ACGTGGC12300.018.60136232
CGTGGCC12000.018.5158633
CACACGT12600.018.33212
GTCACGT12550.018.23028229
CACGTCT13050.017.868314
ACGTCTG13000.017.76780915
CGTCTGA13100.017.4642516
GGCCTTA13350.015.98479136
AGCACAC15150.015.10176410
TATCTCG13800.014.82533341
TCCAGTC15850.014.71186325
GAACTCC16000.014.57372721
GAGCACA16350.014.5315849
CAGTCAC16350.014.53147727
Produced by FastQC (version 0.11.2)