FastQCFastQC Report
Wed 15 Nov 2017
HVH7LAFXX_n01_vs18_171115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH7LAFXX_n01_vs18_171115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7774028
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGCT6600.09.99960731
TCACGGC6250.09.85567630
GTCACGG5900.09.69453429
GCACACG6650.08.93174911
AGTCACG7550.08.44999928
CGGCTAC7150.08.30752633
GATCGGA8250.08.0028261
ACACGTC9100.07.735611413
TGGGGGG8101.8189894E-127.6072815
ACGTCTG10450.06.73623115
CACACGT9304.5474735E-116.623097412
AGGGGGG7007.97063E-86.6023311
CATCTCG10057.2759576E-126.567201639
ACGGCTA10107.2759576E-126.534522532
GAACTCC12350.06.41237321
CACGTCT11203.6379788E-126.285143414
CGTATGC10804.5474735E-116.111027744
AGCACAC11058.367351E-115.97253910
CGTCTGA11653.3105607E-105.66472216
TTGGGGG8553.3320412E-65.40506944
Produced by FastQC (version 0.11.2)