FastQCFastQC Report
Wed 15 Nov 2017
HVH7LAFXX_n01_vs17_171115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH7LAFXX_n01_vs17_171115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7062646
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG11650.017.75043311
CACGTCT12500.017.246414
ACACGTC12500.017.07102213
GATCGGA12800.016.5068861
ACGTCTG12800.016.32649615
CACACGT12600.016.23716412
CGTCTGA13500.015.9688916
CACTAGC13750.015.83976631
CAGTCAC14700.014.36693127
TCCAGTC16250.014.21406725
CGTATGC12650.013.91273944
AGCACAC16000.013.88710
ACTAGCT16700.013.56866232
GAGCACA16150.013.3493649
TATCTCG13700.013.00729239
CCAGTCA17150.012.4422626
TCGGAAG17200.012.4099563
TCACTAG18100.012.39778230
GAACTCC17600.011.99888921
CTAGCTT18700.011.88192433
Produced by FastQC (version 0.11.2)