FastQCFastQC Report
Wed 15 Nov 2017
HVH7LAFXX_n01_vs16_171115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH7LAFXX_n01_vs16_171115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9587629
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG9350.011.29362911
AGTCACG8650.011.19032628
CACACGT10950.09.84406712
CGATCAG10400.09.7304533
ACACGTC10650.09.2951313
ACGTCTG11900.08.68824715
CACGTCT11200.08.64203314
CACGATC12100.08.36340531
CGTATGC12900.08.35653244
GATCTCG12150.08.329205539
GATCGGA13100.08.2317861
GTCACGA12350.08.01597329
CAGTCAC14400.07.63859827
AGGGGGG16350.07.5377121
CGTCTGA13150.07.527798716
TCGTATG16100.07.51532143
TCCAGTC15200.07.380951425
CTCGTAT16150.07.219878742
CCAGTCA15500.07.096393626
AGCACAC15050.06.87020610
Produced by FastQC (version 0.11.2)