FastQCFastQC Report
Wed 15 Nov 2017
HVH7LAFXX_n01_vs15_171115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH7LAFXX_n01_vs15_171115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9615114
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG13400.018.22276911
ACACGTC14500.017.14360413
CACACGT15200.015.91999912
ACGTCTG15450.015.66182415
CGTCTGA16050.015.21339216
GTCACAC16850.014.75296229
CACGTCT17300.014.36861814
GAACTCC19500.013.19864621
GATCGGA16850.013.191971
CAGTCAC19400.013.04050927
AGCACAC19900.012.60237810
TCCAGTC20450.012.37062625
GAGCACA20350.012.2156629
CCAGTCA21000.012.0467626
AGAGCAC21900.011.3512018
AGTCACA22900.011.33561228
TCGGAAG22400.011.1998223
TCACACT24600.010.82081530
ACTCCAG24800.010.46683223
CACACTT27550.09.34278331
Produced by FastQC (version 0.11.2)