FastQCFastQC Report
Wed 15 Nov 2017
HVH7LAFXX_n01_vs14_171115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH7LAFXX_n01_vs14_171115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7999388
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT6900.011.15872612
GCACACG6800.010.3526211
ACACGTC7700.09.42786713
CTTAGGC7900.09.18970333
CACGTCT8500.08.281630514
GATCGGA8800.08.2530461
ACTTAGG8600.08.18599832
CATCTCG9450.08.14799839
ACGTCTG8550.07.97591215
CGTCTGA9200.07.890616416
TAGGCAT11600.07.20680835
TTAGGCA12650.06.956393734
CGTATGC10450.06.94715344
TGGGGGG9355.0931703E-116.59017325
AGGCATC14300.06.307654436
CTCGTAT12250.06.285598342
GAACTCC13150.06.189574221
TCCAGTC12900.06.139037625
GCATCTC13500.06.02955638
CAGTCAC12205.456968E-125.95048627
Produced by FastQC (version 0.11.2)