FastQCFastQC Report
Wed 15 Nov 2017
HVH7LAFXX_n01_vs13_171115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH7LAFXX_n01_vs13_171115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6199096
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG7400.018.72842611
CACACGT8500.016.82235712
ACGTCTG9050.016.04269415
ACACGTC8950.015.73074713
CACGTCT9100.015.47120114
GATCTCG8650.015.26003639
GATCGGA9350.014.8305111
CGTCTGA9750.014.6652916
CGATCTC9100.014.50542238
CACGATC9000.014.42214936
CATCACG10150.013.00412533
CAGTCAC11250.012.7104327
CCAGTCA11550.012.57065526
AGCACAC11550.012.18972610
TCCAGTC11600.012.13698825
GAGCACA12250.012.0319129
TCACGAT11600.011.75799235
AGTCACA12150.011.58785628
GAACTCC13200.011.4989221
CGTATGC11600.011.37888544
Produced by FastQC (version 0.11.2)