FastQCFastQC Report
Wed 15 Nov 2017
HVH7LAFXX_n01_vs12_171115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH7LAFXX_n01_vs12_171115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5522417
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT702181.2715084717434413TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG86450.041.91316628
GCACACG87550.041.83773811
GATCGGA85350.041.6181871
ACACGTC88000.041.54804213
CACACGT88150.041.4777212
CGTCTGA88300.041.25590516
ACGTCTG88650.041.0930215
GTCACGT88150.041.07990329
CACGTCT89550.040.80322314
CACGTGA88950.040.66097331
TCACGTG88950.040.61150430
AGCACAC91100.040.3043510
CGATCTC81850.040.182140
GAACTCC91000.039.88642521
CAGTCAC90900.039.8613127
GAGCACA92450.039.7630469
GATCTCG83000.039.70523541
CTCGTAT86400.039.6446844
TCCAGTC91750.039.4415725
CCAGTCA92700.039.06286626
Produced by FastQC (version 0.11.2)