FastQCFastQC Report
Wed 15 Nov 2017
HVH7LAFXX_n01_vs11_171115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH7LAFXX_n01_vs11_171115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7441199
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT792711.0652987509136633TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC95600.041.46673233
GCACACG99050.041.22091711
ACGTCCG97150.040.94238732
GATCGGA99900.040.4482571
CACACGT101550.040.14113612
ACACGTC101700.040.08193213
CCGCACA97250.040.0401136
CACGTCC99800.040.03104831
AGTCACG100050.040.01978328
GTCCGCA98750.040.0103334
ACGTCTG102150.039.7758815
GTCACGT101050.039.6237429
CACGTCT102950.039.57362714
AGCACAC104150.039.2660510
CGTCTGA103800.039.1859916
TCACGTC102250.039.18023330
TCCGCAC101100.039.0803235
GAGCACA105350.039.027349
CATCTCG96450.038.8893741
TCCAGTC103750.038.8030525
Produced by FastQC (version 0.11.2)