FastQCFastQC Report
Wed 15 Nov 2017
HVH7LAFXX_n01_vs10_171115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH7LAFXX_n01_vs10_171115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8264776
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT1164121.408531822278063TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG148850.042.13614335
GTCCCGA146550.041.7176336
GCACACG156200.041.65939711
GATCGGA155450.041.581321
CCCGTCC151750.041.5773533
ACCCGTC151750.041.53436332
CCGTCCC151600.041.47337334
ACACGTC157250.041.38072613
TCACCCG152750.041.3625230
CCCGATC134550.041.23591638
CACCCGT153700.041.06392331
CACACGT158850.041.01931812
ACGTCTG158400.040.92753215
CACGTCT159100.040.81659314
CCGATCT135500.040.7187739
CGATCTC136950.040.48040840
GTCACCC156600.040.4299129
CGTCTGA160450.040.4046216
AGCACAC161350.040.38424310
TCCCGAT145350.040.33658237
Produced by FastQC (version 0.11.2)