FastQCFastQC Report
Fri 30 Mar 2018
HVH3LAFXX_n02_ds774-5-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH3LAFXX_n02_ds774-5-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6785118
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1573882.3196059375828098No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG463840.6836137558698316Illumina Single End PCR Primer 1 (100% over 58bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG82250.044.03725466
GAGCACA43650.036.90757416
AGAGCAC46750.035.19865415
GCACACG23550.034.85793318
AAGGGGG104950.034.31532367
CCGTATC111050.033.3573649
GCCGTAT111500.033.31479648
ACACGAG14150.032.9134220
CACACGA14500.032.8293219
AGCACAC29550.032.68280417
CGCCGTA117100.031.51868247
CGTATCA118900.031.24276250
AGGGGGG121000.030.53332968
TCGCCGT121300.030.26030546
AAAAGGG121850.029.9803965
GTATCAT126950.029.31554251
GTCGCCG127550.028.86251345
GGTCGCC130100.028.29972344
GTGGTCG136400.026.99262242
CACGAGA17300.026.7220921
Produced by FastQC (version 0.11.2)