FastQCFastQC Report
Fri 30 Mar 2018
HVH3LAFXX_n02_ds774-05-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH3LAFXX_n02_ds774-05-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7050944
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2053152.911879600802389No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG522520.7410638915867152Illumina Single End PCR Primer 1 (100% over 58bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGA45150.055.5422319
GAGCACA127350.053.12051416
ACACGAG47450.052.9260620
AGAGCAC133300.052.485215
GCACACG82400.051.48690418
CACGAGA47850.051.18922821
AGCACAC96600.049.3818117
CACGTAG29950.046.5357521
ACACGTA31850.043.54025720
AAGAGCA198750.042.2839314
AAAGGGG92000.042.07395666
GCACAAG43050.040.14056418
ACGAGAT62500.039.08007422
CACACGT45350.036.9662719
AGCACAA50650.034.73063317
GCGCGCG9550.034.3114618
CGAGATC73850.034.19476723
CGTAGAT42200.034.0896623
AAGGGGG113550.033.99783767
GTGACTG22050.033.985091
Produced by FastQC (version 0.11.2)