FastQCFastQC Report
Fri 30 Mar 2018
HVH3LAFXX_n02_ds774-023-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH3LAFXX_n02_ds774-023-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5439123
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1499232.756381865238201No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG213580.3926735982988434Illumina Single End PCR Primer 1 (100% over 58bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG21350.034.7799261
AAAGGGG48650.031.48357866
TGACTGG23950.031.275922
GACTGGA25350.030.628413
CCGTATC54000.029.18813749
CGCCGTA54750.028.72874547
GCCGTAT55200.028.61609348
CGTATCA59400.026.4773450
TCGCCGT59600.026.21970746
AAGGGGG60600.025.5598167
GGTCGCC61450.025.38004744
GTCGCCG62450.025.02566745
CTGGAGT32900.024.4343015
CTCGGTG65200.024.23716238
CACACGA10050.024.02657719
GTATCAT67700.023.84301451
ACTGGAG34300.023.73944
GTGGTCG66550.023.6940942
ATCTCGG66950.023.65255436
TCTCGGT68400.023.1513637
Produced by FastQC (version 0.11.2)