FastQCFastQC Report
Fri 30 Mar 2018
HVH3LAFXX_n02_ds774-014-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH3LAFXX_n02_ds774-014-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9192176
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG175506419.093019976989126Illumina Single End PCR Primer 1 (100% over 58bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1333821.4510383613194524No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGG183980.20014847409362047Illumina Single End PCR Primer 1 (100% over 58bp)
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA176280.19177178504850212Illumina Single End PCR Primer 1 (100% over 58bp)
GTGACTGGAGTTCAGACGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATC173640.18889977737589012Illumina Single End PCR Primer 1 (100% over 55bp)
GTGACTGGAGTTCAGACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCAT171780.18687631742473165Illumina Single End PCR Primer 1 (100% over 57bp)
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA142010.15449007938925452Illumina Single End PCR Primer 1 (100% over 58bp)
GTGACTGGAGTTCAGACGTGTGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT131260.14279535117691391Illumina Single End PCR Primer 1 (100% over 52bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAATGGGGGG126490.13760615549571723Illumina Single End PCR Primer 1 (100% over 58bp)
AGATCGGAAGAGCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAA115530.12568297212760068Illumina Single End PCR Primer 1 (100% over 58bp)
GTGACTGGAGTTCAGACGTGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTA114100.12412730130493585Illumina Single End PCR Primer 1 (100% over 53bp)
ACACTCTTTCCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA110500.12021092720591947Illumina Single End PCR Primer 1 (100% over 58bp)
AGATCGGAAGAGCGTCGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCA108300.11781758747874278Illumina Single End PCR Primer 1 (100% over 56bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGG104740.11394472864749326Illumina Single End PCR Primer 1 (100% over 58bp)
GTGACTGGAGTTCAGACGTGTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG96630.10512200810776469Illumina Single End PCR Primer 1 (100% over 51bp)
AGATCGGAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA93950.10220648516738583Illumina Single End PCR Primer 1 (100% over 58bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA816800.064.4422916
AGAGCAC832250.064.39944515
CACACGA238150.064.2938319
GCACAAG341000.063.88777518
AGCACAA341800.063.607417
AAGAGCA1186300.063.536314
ACACGAG242550.063.1177520
GAGCAAG305500.062.98062516
GTGACTG153250.062.894471
AAGGGGG2066600.062.37785367
AGAGCAA316900.062.2292615
AGGGGGG2083100.061.82150368
AGCACAC461800.061.79331217
CACGAGA245200.061.3298821
TGACTGG159100.060.8906632
AAAGGGG2122350.060.8872366
GCACACG401450.060.84055318
CACAAGA357950.060.54516219
AAAAGGG2170450.059.7798165
AGAGAGA436850.059.59490215
Produced by FastQC (version 0.11.2)