FastQCFastQC Report
Fri 30 Mar 2018
HVH3LAFXX_n02_ds774-005-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH3LAFXX_n02_ds774-005-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13076498
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG174120413.315522244564256Illumina Single End PCR Primer 1 (100% over 58bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2319511.7738006001300959No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAGGGGGG336350.25721718460095355Illumina Single End PCR Primer 1 (100% over 58bp)
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA200770.1535349907903477Illumina Single End PCR Primer 1 (100% over 58bp)
GTGACTGGAGTTCAGACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCAT165430.12650940641752859Illumina Single End PCR Primer 1 (100% over 57bp)
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA158890.12150806737400181Illumina Single End PCR Primer 1 (100% over 58bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGG153400.11730969560810547Illumina Single End PCR Primer 1 (100% over 58bp)
GTGACTGGAGTTCAGACGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATC151910.11617024680461083Illumina Single End PCR Primer 1 (100% over 55bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACAAG521750.064.0528818
AGCACAA524950.063.67605617
AGAGCAC1235550.063.50860615
GAGCACA1208550.063.4501616
CACACGA331300.063.2862919
GAGCAAG432000.063.0391516
AAGAGCA1741350.062.61325514
ACACGAG336750.062.2744520
AGAGCAA452300.061.9121615
AAGGGGG2082350.061.50135467
CACAAGA545750.061.12910519
GTGACTG158450.061.0035741
CACGAGA340000.060.79049721
AAAGGGG2127550.060.36176766
AGCACAC664200.059.6894817
ACACGTA167150.059.37708320
AGAGAGA551950.059.31621615
AGGGGGG2160050.059.26282568
AGCAAGA462100.059.0426717
AAAAGGG2183700.059.04031865
Produced by FastQC (version 0.11.2)