FastQCFastQC Report
Fri 30 Mar 2018
HVH3LAFXX_n02_ds395-5-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH3LAFXX_n02_ds395-5-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10846902
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1159711.0691624207538706No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG911290.8401385022193434Illumina Single End PCR Primer 1 (100% over 58bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG155700.044.02526566
AAGGGGG169700.040.3525967
AGGGGGG181350.037.87463468
GAGCACA86300.037.57697716
AGAGCAC87900.037.5598515
CACACGA27700.036.48668719
GCACACG50550.036.30003418
ACACGAG28200.035.47525820
AGCACAC63500.032.3731617
AAAAGGG215300.032.11060365
CGGGGAG19450.029.4762821
CACGAGA33500.029.35018321
CGGGGTT19500.029.2235891
CGGGGAC14200.029.1861691
GGGGGAT34700.027.6693861
CACGTAG22700.026.74838621
CGGGGTG16650.026.5509431
AAAAAGG278250.025.39215564
GTGACTG31200.024.6850761
CGGGGAT21600.024.14391
Produced by FastQC (version 0.11.2)