FastQCFastQC Report
Fri 30 Mar 2018
HVH3LAFXX_n02_ds395-023-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH3LAFXX_n02_ds395-023-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7895181
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG2850643.61060753388681Illumina Single End PCR Primer 1 (100% over 58bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1236931.5666898580285873No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGA90650.059.77926619
GAGCACA305450.059.05835316
AGAGCAC314500.058.65291215
ACACGAG93650.058.16411220
CACGAGA94350.057.29855721
AAGGGGG377450.055.55044667
AAAGGGG379950.055.3211266
GCACAAG121600.055.1112318
GCACACG166250.054.8322918
CACGTAG58500.054.72788221
AGCACAC195100.054.14970817
AAGAGCA442300.054.09225514
AGCACAA127300.052.77970517
AGGGGGG401000.052.27152368
ACACGTA60850.051.9296820
GTGACTG52750.051.3976671
AAAAGGG421100.050.18515865
GAGCAAG96500.049.6566916
TGACTGG56000.049.128342
ACGAGAT113750.047.4959922
Produced by FastQC (version 0.11.2)