FastQCFastQC Report
Fri 30 Mar 2018
HVH3LAFXX_n02_ds131-5-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH3LAFXX_n02_ds131-5-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10815297
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2662042.461365600963154No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG1154091.0670904368137093Illumina Single End PCR Primer 1 (100% over 58bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA98650.048.46799516
GCACACG53950.048.0132418
AGAGCAC101400.047.6642915
AAAGGGG186850.047.26523666
AAGGGGG192550.045.88397667
AGCACAC69900.042.8800417
CACACGA35150.040.82486319
ACACGAG36400.039.99410620
AGGGGGG225900.039.26239468
AAAAGGG245050.036.50402565
CACGAGA40650.035.90053621
CACGTAG28850.033.72286221
AAGAGCA186000.032.7546314
CACACGT36450.031.3247219
CCGTATC304000.029.86144349
CGTATCA305550.029.82380550
GCCGTAT306200.029.7474448
AAAAAGG305350.029.67967264
ACACGTA31750.029.66223320
GTATCAT319050.028.56239551
Produced by FastQC (version 0.11.2)