FastQCFastQC Report
Fri 30 Mar 2018
HVH3LAFXX_n02_ds131-5-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH3LAFXX_n02_ds131-5-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10876961
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2100521.9311644125597212No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG473190.4350387943838357Illumina Single End PCR Primer 1 (100% over 58bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG33150.041.09847318
AGAGCAC65350.039.1189115
GAGCACA64000.038.7035316
AAAGGGG102800.035.2369566
AGCACAC45100.033.4972917
CACACGA23050.031.72495819
AAGGGGG114800.031.55379167
ACACGAG23550.031.19958320
GCCGTAT135050.027.60585848
CCGTATC135450.027.57602149
CGCCGTA137800.027.08296247
CACGAGA26650.026.79669621
TCGCCGT145000.025.83627746
CGTATCA145500.025.6722350
AGGGGGG147050.025.31410268
GTCGCCG154150.024.30750745
GGTCGCC158050.023.75398644
CACACGT26050.023.70180519
CACGTAG22050.023.62525221
GTATCAT164050.023.08480651
Produced by FastQC (version 0.11.2)