FastQCFastQC Report
Fri 30 Mar 2018
HVH3LAFXX_n02_ds131-05-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH3LAFXX_n02_ds131-05-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12763578
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2351651.8424692511770602No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG1426511.117641150467369Illumina Single End PCR Primer 1 (100% over 58bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG226150.047.39739666
AAGGGGG247350.043.34915567
GCACACG46200.041.6592818
AGAGCAC97600.041.60201315
GAGCACA97150.041.2265616
AGGGGGG269300.040.02168
ACACGAG30750.037.02040520
AGCACAC61550.036.985417
AAAAGGG293650.036.89021365
CACACGA31200.036.7058619
CCGTATC327950.033.87402749
GCCGTAT331900.033.6576748
CGTATCA335700.033.1134750
CGCCGTA344100.032.24649447
CACGAGA35850.031.56368421
GTATCAT352650.031.52225351
TCGCCGT355600.031.14985546
AAAAAGG353450.030.98113364
GTCGCCG371000.029.92603745
GGTCGCC379600.029.19615244
Produced by FastQC (version 0.11.2)