FastQCFastQC Report
Fri 30 Mar 2018
HVH3LAFXX_n02_ds131-005-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH3LAFXX_n02_ds131-005-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9304982
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2481632.6669906508148No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG714410.7677715013312224Illumina Single End PCR Primer 1 (100% over 58bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA110750.047.222516
AGAGCAC113350.046.44416815
ACACGAG35450.045.1514720
GCACACG63700.044.77910618
CACACGA37150.043.36131719
AGCACAC76950.042.7169917
CACGAGA38850.040.9373521
AAAGGGG133850.040.46368466
AAGGGGG140750.038.406567
CACGTAG28600.036.79117621
AAGAGCA186600.035.99585314
GCACAAG50850.034.93217518
ACACGTA30150.034.66773220
GTGACTG37700.033.614921
AGGGGGG162850.033.55492468
TGACTGG41150.031.5384712
GACTGGA42400.031.0083623
AAAAGGG176850.030.95679765
ACGAGAT53750.029.33239722
CGAGATC56050.028.86751623
Produced by FastQC (version 0.11.2)