FastQCFastQC Report
Fri 30 Mar 2018
HVH3LAFXX_n01_ds774-5-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH3LAFXX_n01_ds774-5-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6785118
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG441780.6511014252073435TruSeq Adapter, Index 11 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG108300.032.87928458
TGCCGTC113850.031.55132349
GCCGTCT113650.031.3034650
TGCTTGA113900.031.2909659
ATGCCGT118200.030.50649848
CCGTCTT117400.030.42135651
GCTTGAA124200.029.16650260
CGTCTTC126700.028.54243352
TATGCCG127750.028.2529847
GTATGCC130150.027.65143246
CATCTCG141050.025.1165340
CTTCTGC147650.024.51520755
TCTGCTT148900.024.4010257
CGTATGC149500.024.16015845
CACGGCT153050.023.75649532
CGGCTAC153650.023.52901634
CTCGTAT152500.023.5253143
GTCTTCT155100.023.42681753
TCACGGC155350.023.40477231
GGCTACA153650.023.39430835
Produced by FastQC (version 0.11.2)