FastQCFastQC Report
Fri 30 Mar 2018
HVH3LAFXX_n01_ds774-05-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH3LAFXX_n01_ds774-05-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7050944
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG469170.6654002641348449TruSeq Adapter, Index 12 (100% over 63bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT39700.032.0852551
ACGTGTG70600.030.4616171
TGCTTGA217550.029.71811965
GCTTGAA218850.029.63617366
CTGCTTG223850.028.85050464
CGTGTGC118400.028.2806661
GTGTGCT131200.025.6496732
CGTCTTC260800.024.9100858
CTTGAAA264500.024.71691967
TCTGCTT266650.024.36202663
CCGTCTT270250.024.0269557
GCCGTCT272050.023.80574856
CTTCTGC277750.023.41309761
GTCTTCT280200.023.22052859
TCTTCTG283500.023.0355860
TGAAAAA292500.022.59854369
TGCCGTC286200.022.56928855
TTGAAAA296200.022.16483968
TTCTGCT294650.022.07021162
ATGCCGT297050.021.82623954
Produced by FastQC (version 0.11.2)