FastQCFastQC Report
Fri 30 Mar 2018
HVH3LAFXX_n01_ds774-023-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH3LAFXX_n01_ds774-023-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5439123
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG180320.331524034297441TruSeq Adapter, Index 13 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT54750.026.02794850
CTGCTTG55350.025.7439158
TGCCGTC56600.025.29843749
ATGCCGT58650.024.35512548
CCGTCTT59800.024.00325651
TGCTTGA60050.023.95748159
GTGACTG13850.023.675961
GCTTGAA65100.022.47020160
GTATGCC65100.022.20624246
TATGCCG64650.022.20152747
CGTCTTC65600.022.09141252
TGACTGG15150.021.8689772
GACTGGA15200.021.56843
CACAGTC81200.019.62764232
CTCGTAT73450.019.11546143
GGAGTTC17500.018.7273247
CTTCTGC78250.018.6959555
TCACAGT87800.018.34866731
GTCACAG87250.018.14802730
CGTATGC80100.018.13208445
Produced by FastQC (version 0.11.2)