FastQCFastQC Report
Fri 30 Mar 2018
HVH3LAFXX_n01_ds774-014-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH3LAFXX_n01_ds774-014-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9192176
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG162864417.717719939217876TruSeq Adapter, Index 14 (98% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG260320.2831973626266512RNA PCR Primer, Index 14 (97% over 43bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG237370.25823047774542174No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCA218380.23757160437311034TruSeq Adapter, Index 14 (97% over 39bp)
AGAATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA165450.17999002630062783RNA PCR Primer, Index 14 (100% over 43bp)
CACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTC161040.17519246802933278TruSeq Adapter, Index 14 (98% over 54bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG158750.17270121894968068TruSeq Adapter, Index 14 (98% over 63bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTCTTCTGCTTG157610.1714610338183255TruSeq Adapter, Index 14 (98% over 63bp)
CGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTCTT155160.168795723667606TruSeq Adapter, Index 14 (98% over 56bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA152610.16602162534746942TruSeq Adapter, Index 14 (98% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAC140150.15246661943809606TruSeq Adapter, Index 14 (98% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG128520.1398145553348848RNA PCR Primer, Index 14 (97% over 43bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTCTTCTGCTTGA127130.13830239977998682TruSeq Adapter, Index 14 (98% over 63bp)
GCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCC116070.12627042824245313TruSeq Adapter, Index 14 (98% over 51bp)
CACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCG102050.11101832689017269TruSeq Adapter, Index 14 (98% over 52bp)
GGGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTCTTCTGCTT100250.1090601398406645TruSeq Adapter, Index 14 (98% over 62bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAT95600.10400148996276834TruSeq Adapter, Index 14 (98% over 63bp)
CGCTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTCTTCTGC95580.10397973232888491TruSeq Adapter, Index 14 (98% over 60bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG2005000.063.78457669
GAAAAAA2384650.055.35542764
GAATCGG31600.052.752682
AGAATCG35500.047.3565061
TGAAAAA2780450.047.19509563
TTGAAAA2779050.047.01527462
CTTGAAA2786750.046.82419661
GCTTGAA2818700.046.14875860
TGCTTGA2957150.043.8206159
GACGTGT91400.043.4340861
ACGTGTG244050.043.057111
CTGCTTG3050800.042.45378558
TCTGCTT3165100.040.9466357
TTCTGCT3183900.040.7475856
CTTCTGC3231900.040.27673355
TCTTCTG3273250.039.6981454
GTCTTCT3313850.039.27424653
AAAAAGG132250.038.66192269
TGTAGGG17700.038.42131
CGTCTTC3407050.038.29834452
Produced by FastQC (version 0.11.2)