FastQCFastQC Report
Fri 30 Mar 2018
HVH3LAFXX_n01_ds774-005-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH3LAFXX_n01_ds774-005-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13076498
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG165388112.647736419949746TruSeq Adapter, Index 2 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG291890.2232172558738586No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG195960.14985663592805964TruSeq Adapter, Index 2 (100% over 63bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTG189020.14454940458829266TruSeq Adapter, Index 2 (100% over 63bp)
AGAATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA181140.1385233263523613TruSeq Adapter, Index 2 (98% over 63bp)
CGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTT155970.11927505361144858TruSeq Adapter, Index 2 (100% over 56bp)
AGATGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG155460.11888504093374236TruSeq Adapter, Index 2 (100% over 59bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAT148180.11331780114217124TruSeq Adapter, Index 2 (100% over 63bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGA147870.1130807346125851TruSeq Adapter, Index 2 (100% over 63bp)
CACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTC147670.11292778846446502TruSeq Adapter, Index 2 (100% over 54bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA132990.10170154119245076TruSeq Adapter, Index 2 (100% over 63bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG2045950.061.98469
GAAAAAA2416850.054.05130864
GAATCGG35700.049.2115252
TTGAAAA2758250.046.92247862
TGAAAAA2775350.046.91111463
CTTGAAA2766200.046.72526661
GCTTGAA2787400.046.23480660
GACGTGT201350.044.1468161
TGCTTGA2929250.043.76519459
AGAATCG41650.042.684091
CTGCTTG3044050.042.0701458
TCTGCTT3188400.040.17738357
TTCTGCT3208050.039.8790356
CTTCTGC3253600.039.475155
TCTTCTG3308800.038.78005254
GTCTTCT3351950.038.3024553
CGTCTTC3450950.037.3220552
ACGTGTG431750.037.018811
AAAAAGG169900.033.9935669
CGTGTGC837050.033.610891
Produced by FastQC (version 0.11.2)