FastQCFastQC Report
Fri 30 Mar 2018
HVH3LAFXX_n01_ds395-5-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH3LAFXX_n01_ds395-5-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10846902
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG851590.7850997455310281TruSeq Adapter, Index 5 (100% over 63bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA245350.027.8699460
TGCTTGA254850.026.61493159
CTGCTTG280750.024.01315758
AAAAAAG289850.023.80571269
CTTGAAA293400.023.61141661
GAAAAAA331900.021.40324264
TGAAAAA338850.020.87242163
TTGAAAA347200.020.34045262
CGTGTGC62700.019.3785761
ACGTGTG49350.018.4656051
TCTGCTT374400.018.2096557
CTTCTGC413400.016.65140755
TTCTGCT421250.016.30789456
GACGTGT36400.016.1210861
GTGTGCT76550.016.0955452
TCTTCTG454900.015.1777654
CCGTCTT450350.015.13210951
CGTCTTC455050.015.05922652
GTCTTCT464100.014.89932753
GCCGTCT466550.014.60654350
Produced by FastQC (version 0.11.2)