FastQCFastQC Report
Fri 30 Mar 2018
HVH3LAFXX_n01_ds395-023-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH3LAFXX_n01_ds395-023-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7895181
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG2683023.3983008115963393TruSeq Adapter, Index 15 (98% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAA119090.15083884713979326TruSeq Adapter, Index 15 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG441550.046.5767469
GCTTGAA503200.041.3832660
TGCTTGA511500.040.63106559
CTGCTTG522800.039.7094258
GAAAAAA545450.038.96254764
ACGTGTG112200.038.520481
CTTGAAA545800.038.36818361
TTGAAAA570550.036.77659662
TGAAAAA575550.036.5712363
TCTGCTT571450.036.35339757
CTTCTGC575750.036.27618455
TTCTGCT586950.035.49489256
CGTCTTC592250.035.26060552
TCTTCTG595650.035.09899554
GTCTTCT599750.034.8479953
CGTGTGC207550.032.150621
CCGTCTT651100.031.99936151
GCCGTCT661150.031.51274550
TGCCGTC732800.028.42631749
GTGTGCT235200.028.3958662
Produced by FastQC (version 0.11.2)