FastQCFastQC Report
Fri 30 Mar 2018
HVH3LAFXX_n01_ds131-5-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH3LAFXX_n01_ds131-5-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10815297
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG1024670.947426594017714TruSeq Adapter, Index 8 (100% over 63bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG251150.033.69941758
TGCTTGA258500.032.85832259
GCTTGAA267250.032.55646560
GCCGTCT286050.029.7592150
CCGTCTT292650.029.217951
AAAAAAG309250.028.9065169
TGCCGTC298600.028.86614449
ATGCCGT307100.028.24656148
TCTGCTT305800.027.8351657
CGTCTTC310750.027.5826952
CTTGAAA321600.027.37628461
CTTCTGC318050.026.87303755
TATGCCG324750.026.70087247
GTCTTCT323850.026.07271453
GTATGCC341500.025.61115846
TTCTGCT332200.025.55145556
ACGTGTG49100.025.3790041
TCTTCTG342100.024.91334754
GAAAAAA367100.024.77301664
CGTGTGC72050.024.3855551
Produced by FastQC (version 0.11.2)