FastQCFastQC Report
Fri 30 Mar 2018
HVH3LAFXX_n01_ds131-5-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH3LAFXX_n01_ds131-5-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10876961
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG435220.40013014664665986TruSeq Adapter, Index 7 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG124450.028.11142258
GCCGTCT134800.026.441550
TGCCGTC136200.026.37177349
CCGTCTT137900.026.32274451
TGCTTGA135250.026.14630759
ATGCCGT140250.025.60999748
TATGCCG150050.024.12143747
GCTTGAA153200.023.66789860
GTATGCC157100.023.05933846
ACGTGTG34950.022.4198191
CGTCTTC166300.021.9519552
CGTGTGC47250.020.3824231
CGTATGC181600.019.9276245
CATCTCG177650.019.82596640
TCTGCTT182900.019.56179857
CTCGTAT187650.018.89819743
CTTCTGC193250.018.87222955
GTCTTCT196100.018.56318753
TCGTATG198100.018.09359444
ATCTCGT197550.017.84636141
Produced by FastQC (version 0.11.2)