FastQCFastQC Report
Fri 30 Mar 2018
HVH3LAFXX_n01_ds131-05-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH3LAFXX_n01_ds131-05-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12763578
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG1378811.0802691847066708TruSeq Adapter, Index 9 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG304750.035.75280858
TGCTTGA313350.034.91319359
GCTTGAA324900.033.9371260
GCCGTCT327600.033.34527250
CCGTCTT332400.032.8226251
TGCCGTC335850.032.59769449
ATGCCGT343300.031.88011248
CGTCTTC352700.031.08022152
TATGCCG365100.030.00479547
TCTGCTT372200.029.38515957
GTATGCC375200.029.17744646
CTTCTGC378550.029.11234755
GTCTTCT390700.028.11008653
CTTGAAA398650.027.83185661
TCTTCTG405550.027.1656554
AAAAAAG402050.027.09095869
CGTATGC407050.026.93417445
TTCTGCT408850.026.89543256
GATCTCG399000.026.53358840
CTCGTAT412550.026.16421743
Produced by FastQC (version 0.11.2)