FastQCFastQC Report
Fri 30 Mar 2018
HVH3LAFXX_n01_ds131-005-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH3LAFXX_n01_ds131-005-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9304982
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG665220.714907347483316TruSeq Adapter, Index 1 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG163500.032.11780558
TGCTTGA169450.030.96883859
GCTTGAA172850.030.63846260
GCCGTCT189950.027.81001350
CCGTCTT194300.027.16993551
CGTCTTC197750.026.87040352
ACGTGTG52850.025.7343181
CTTCTGC207950.025.65195855
TCTGCTT206600.025.53456557
TGCCGTC208800.025.43103849
CGTGTGC78100.025.4143051
GTCTTCT217200.024.75013753
ATGCCGT215400.024.60349748
CTTGAAA227200.023.64330961
TTCTGCT229150.023.24813556
TCTTCTG235650.022.76842154
TATGCCG240650.022.02199447
GTGTGCT92750.021.5451242
GTATGCC250900.021.17642646
AAAAAAG262300.020.25606569
Produced by FastQC (version 0.11.2)