FastQCFastQC Report
Wed 28 Sep 2022
HVH3HBGXM_n01_YZ09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH3HBGXM_n01_YZ09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4572595
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC288610.6311733271807366TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC163020.35651528289734824TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTATGC93780.20509141964245686TruSeq Adapter, Index 5 (97% over 41bp)

[OK]Adapter Content

Adapter graph