FastQCFastQC Report
Thu 22 Feb 2024
HVH3FDRX3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH3FDRX3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences902107327
Sequences flagged as poor quality0
Sequence length10
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGCTCATT39588958243.884975784039945No Hit
TGTTAAACCG22468304924.906465370056793No Hit
AGGTAACACT20998818923.277517288139705No Hit
AGATCTCGGT416836224.620694317894616Illumina Single End PCR Primer 1 (100% over 10bp)
GTCTCGTTCG56736300.628930708152867No Hit
GAGGTATGTG33647310.3729856636005352No Hit
GGGGGGGGGG17199570.1906599080310984No Hit
GAGCTATGTG10338060.11459900269715911No Hit
GTCACGTTCG9395110.10414625531580458No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)