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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2019-07-23, 12:07 based on data in: /beegfs/mk5636/logs/html/HVGW3BGXB/merged

        General Statistics

        Showing 46/46 rows and 4/5 columns.
        Sample Name% Dups% GCLengthM Seqs
        HVGW3BGXB_n01_dpErk_HTO1_5-14hpf
        79.0%
        47%
        28
        22.8
        HVGW3BGXB_n01_dpErk_HTO2_16.5hpf
        86.5%
        47%
        28
        6.7
        HVGW3BGXB_n01_dpErk_WE_16.5hpf_1
        6.4%
        48%
        28
        14.7
        HVGW3BGXB_n01_dpErk_WE_16.5hpf_2
        6.3%
        48%
        28
        14.4
        HVGW3BGXB_n01_dpErk_WE_16.5hpf_3
        6.2%
        48%
        28
        14.0
        HVGW3BGXB_n01_dpErk_WE_16.5hpf_4
        5.6%
        48%
        28
        11.6
        HVGW3BGXB_n01_dpErk_WE_514hpf_E1_1
        2.5%
        47%
        28
        10.7
        HVGW3BGXB_n01_dpErk_WE_514hpf_E1_2
        2.6%
        47%
        28
        10.9
        HVGW3BGXB_n01_dpErk_WE_514hpf_E1_3
        2.7%
        47%
        28
        13.7
        HVGW3BGXB_n01_dpErk_WE_514hpf_E1_4
        2.6%
        47%
        28
        12.1
        HVGW3BGXB_n01_dpErk_WE_514hpf_F1_1
        2.7%
        47%
        28
        12.4
        HVGW3BGXB_n01_dpErk_WE_514hpf_F1_2
        2.6%
        47%
        28
        11.9
        HVGW3BGXB_n01_dpErk_WE_514hpf_F1_3
        2.7%
        47%
        28
        12.5
        HVGW3BGXB_n01_dpErk_WE_514hpf_F1_4
        2.6%
        47%
        28
        12.4
        HVGW3BGXB_n01_dpErk_WE_514hpf_G1_1
        2.6%
        47%
        28
        10.7
        HVGW3BGXB_n01_dpErk_WE_514hpf_G1_2
        2.5%
        47%
        28
        11.0
        HVGW3BGXB_n01_dpErk_WE_514hpf_G1_3
        2.6%
        47%
        28
        11.2
        HVGW3BGXB_n01_dpErk_WE_514hpf_G1_4
        2.7%
        47%
        28
        11.6
        HVGW3BGXB_n01_dpErk_WE_514hpf_H1_1
        2.7%
        47%
        28
        11.4
        HVGW3BGXB_n01_dpErk_WE_514hpf_H1_2
        2.6%
        47%
        28
        11.8
        HVGW3BGXB_n01_dpErk_WE_514hpf_H1_3
        2.7%
        47%
        28
        13.0
        HVGW3BGXB_n01_dpErk_WE_514hpf_H1_4
        2.5%
        47%
        28
        10.1
        HVGW3BGXB_n01_undetermined
        74.4%
        46%
        28
        47.8
        HVGW3BGXB_n02_dpErk_HTO1_5-14hpf
        88.9%
        65%
        110
        22.8
        HVGW3BGXB_n02_dpErk_HTO2_16.5hpf
        89.3%
        77%
        110
        6.7
        HVGW3BGXB_n02_dpErk_WE_16.5hpf_1
        62.8%
        40%
        110
        14.7
        HVGW3BGXB_n02_dpErk_WE_16.5hpf_2
        63.0%
        40%
        110
        14.4
        HVGW3BGXB_n02_dpErk_WE_16.5hpf_3
        62.7%
        40%
        110
        14.0
        HVGW3BGXB_n02_dpErk_WE_16.5hpf_4
        61.2%
        40%
        110
        11.6
        HVGW3BGXB_n02_dpErk_WE_514hpf_E1_1
        55.8%
        39%
        110
        10.7
        HVGW3BGXB_n02_dpErk_WE_514hpf_E1_2
        55.8%
        39%
        110
        10.9
        HVGW3BGXB_n02_dpErk_WE_514hpf_E1_3
        57.4%
        39%
        110
        13.7
        HVGW3BGXB_n02_dpErk_WE_514hpf_E1_4
        57.0%
        39%
        110
        12.1
        HVGW3BGXB_n02_dpErk_WE_514hpf_F1_1
        56.4%
        39%
        110
        12.4
        HVGW3BGXB_n02_dpErk_WE_514hpf_F1_2
        56.6%
        39%
        110
        11.9
        HVGW3BGXB_n02_dpErk_WE_514hpf_F1_3
        57.0%
        39%
        110
        12.5
        HVGW3BGXB_n02_dpErk_WE_514hpf_F1_4
        57.2%
        39%
        110
        12.4
        HVGW3BGXB_n02_dpErk_WE_514hpf_G1_1
        56.1%
        39%
        110
        10.7
        HVGW3BGXB_n02_dpErk_WE_514hpf_G1_2
        55.5%
        39%
        110
        11.0
        HVGW3BGXB_n02_dpErk_WE_514hpf_G1_3
        55.6%
        39%
        110
        11.2
        HVGW3BGXB_n02_dpErk_WE_514hpf_G1_4
        56.6%
        39%
        110
        11.6
        HVGW3BGXB_n02_dpErk_WE_514hpf_H1_1
        56.4%
        39%
        110
        11.4
        HVGW3BGXB_n02_dpErk_WE_514hpf_H1_2
        56.7%
        39%
        110
        11.8
        HVGW3BGXB_n02_dpErk_WE_514hpf_H1_3
        56.8%
        39%
        110
        13.0
        HVGW3BGXB_n02_dpErk_WE_514hpf_H1_4
        55.5%
        39%
        110
        10.1
        HVGW3BGXB_n02_undetermined
        80.9%
        46%
        110
        47.8

        Demultiplexing Report

        Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.

        Showing 23/23 rows and 2/2 columns.
        Sample NameTotal Read CountPortion (%)
        undetermined_library
        47,842,289
        15.0
        dpErk_HTO2_16.5hpf
        6,741,546
        2.1
        dpErk_HTO1_5-14hpf
        22,842,481
        7.2
        dpErk_WE_16.5hpf_1
        14,654,079
        4.6
        dpErk_WE_16.5hpf_2
        14,416,002
        4.5
        dpErk_WE_16.5hpf_3
        13,950,022
        4.4
        dpErk_WE_16.5hpf_4
        11,608,349
        3.6
        dpErk_WE_514hpf_E1_1
        10,668,027
        3.3
        dpErk_WE_514hpf_E1_2
        10,884,044
        3.4
        dpErk_WE_514hpf_E1_3
        13,711,066
        4.3
        dpErk_WE_514hpf_E1_4
        12,120,852
        3.8
        dpErk_WE_514hpf_F1_1
        12,446,909
        3.9
        dpErk_WE_514hpf_F1_2
        11,879,602
        3.7
        dpErk_WE_514hpf_F1_3
        12,488,764
        3.9
        dpErk_WE_514hpf_F1_4
        12,351,654
        3.9
        dpErk_WE_514hpf_G1_1
        10,667,248
        3.3
        dpErk_WE_514hpf_G1_2
        10,967,995
        3.4
        dpErk_WE_514hpf_G1_3
        11,220,335
        3.5
        dpErk_WE_514hpf_G1_4
        11,562,152
        3.6
        dpErk_WE_514hpf_H1_1
        11,410,106
        3.6
        dpErk_WE_514hpf_H1_2
        11,752,017
        3.7
        dpErk_WE_514hpf_H1_3
        12,976,321
        4.1
        dpErk_WE_514hpf_H1_4
        10,101,821
        3.2

        Barcodes of Undetermined Reads

        Barcodes of Undetermined Reads
        We have determined the barcodes of your undetermined reads (reads containing a barcode that you did not encode in your metadata). Here are the top 20 barcodes belonging to the undetermined reads. The full list is available here.

        Showing 20/20 rows and 2/2 columns.
        Sample NameCountFrequency (%)
        GGGGGGGG
        37504829.0
        78.4
        ACCCTCTA
        104069.0
        0.2
        CACTCGAA
        90507.0
        0.2
        CAGGAGCA
        85017.0
        0.2
        GCTGAATA
        78688.0
        0.2
        CACGTCGA
        77040.0
        0.2
        ACCTCCTA
        73121.0
        0.1
        CACCGGAA
        71543.0
        0.1
        TGGAATAA
        68484.0
        0.1
        CAATGAGA
        67678.0
        0.1
        CATGGAGA
        66225.0
        0.1
        CCCCTCCT
        64817.0
        0.1
        GTGCAGCA
        64459.0
        0.1
        ATACTCGA
        63047.0
        0.1
        TGTCAGCC
        62126.0
        0.1
        ATGCTCGA
        61491.0
        0.1
        ACCCTCCA
        57030.0
        0.1
        ATTACTCA
        54811.0
        0.1
        TGTAAACA
        54029.0
        0.1
        TAAGTACA
        53799.0
        0.1

        Run Statistics

        Run Statistics

        Showing 1/1 rows and 4/4 columns.
        Sample NameTotal # of Single-End ReadsTotal # PF Reads% Undetermined % PhiX Aligned
        4.0
        333,929,936
        319,263,681
        15.0
        10.8

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

        loading..

        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

        loading..

        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

        loading..

        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

        loading..

        Sequence Length Distribution

        All samples have sequences of a single length (28bp , 110bp). See the General Statistics Table.

        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

        loading..

        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

        loading..

        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

        loading..

        MultiQC v1.0.dev0 - Written by Phil Ewels, available on GitHub.

        This report uses HighCharts, jQuery, jQuery UI, Bootstrap, FileSaver.js and clipboard.js.