FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47842289
Sequences flagged as poor quality0
Sequence length110
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16039363.3525486207401154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCAT555450.099.142322
AGGACCA573900.096.062851
AGCAGTG947000.082.762892
GTTACCG123250.076.0072255
GACCATC756800.073.8915253
CCATCCA763700.069.258185
AAGCAGT1203950.065.2092741
TGTTACC158350.064.535054
GTATCAA1237250.063.6749849
TCCAATA115200.058.7909978
TCCAACA197050.055.6940468
ATGTTAC192100.055.524483
CATGTTA191900.055.4943852
ACCATCC990650.055.1615684
GGTATCA1456150.054.258278
GCAGTGG1467000.054.0754053
CAGTGGT1520100.052.03744
ATCCAAT133050.051.8500677
AGTGGTA1555250.051.3575745
ACATGTT207400.050.847591
Produced by FastQC (version 0.11.5)