FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n02_dpErk_WE_514hpf_H1_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n02_dpErk_WE_514hpf_H1_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10101821
Sequences flagged as poor quality0
Sequence length110
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC310620.30748911508133037No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA201300.19927100272317239No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT191580.18964897516992235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1018600.096.637561
AGCAGTG1139850.086.233572
GCAGTGG1340700.073.343293
CAGTGGT1429700.068.7095264
AGTGGTA1454050.067.6070565
TGGTATC1497450.065.526517
GTGGTAT1509700.065.07626
GGTATCA1511750.064.9345258
GTATCAA1540550.063.670289
CAACGCA1539950.036.16189212-13
ATCAACG1547900.036.0213410-11
GCAGAGT1555600.035.62029616-17
AGAGTAC1550450.035.5747918-19
ACGCAGA1576900.035.2772314-15
TACATGG1569000.034.5694322-23
AGTACAT1632850.033.6972420-21
CATGGGG465500.033.1581724-25
TCAACGC1531800.032.01730712-13
CATGGGA1040250.031.71301524-25
AACGCAG1565600.031.27015114-15
Produced by FastQC (version 0.11.5)