FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n02_dpErk_WE_514hpf_H1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n02_dpErk_WE_514hpf_H1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12976321
Sequences flagged as poor quality0
Sequence length110
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC392710.30263585495457457No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA251950.19416134973849677No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT243640.18775737745698493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1311050.096.85641
AGCAGTG1464500.086.571822
GCAGTGG1715950.073.827063
CAGTGGT1831350.069.092174
AGTGGTA1857550.068.181865
TGGTATC1907950.066.2319957
GGTATCA1924150.065.708578
GTGGTAT1929150.065.63696
GTATCAA1961650.064.413189
CAACGCA1965200.036.3752312-13
ATCAACG1975200.036.2403310-11
AGAGTAC1975300.035.8712218-19
GCAGAGT1980400.035.87041516-17
ACGCAGA2002300.035.5806114-15
TACATGG1996900.034.8701522-23
AGTACAT2075000.034.03119720-21
CATGGGG582350.033.3776524-25
TCAACGC1951100.032.362712-13
CATGGGA1321100.032.0538924-25
AACGCAG1991050.031.67298914-15
Produced by FastQC (version 0.11.5)