FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n02_dpErk_WE_514hpf_H1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n02_dpErk_WE_514hpf_H1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11752017
Sequences flagged as poor quality0
Sequence length110
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC358460.30501998082541915No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA231970.1973873931598295No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT221990.18889523389899793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1187800.097.06551
AGCAGTG1323200.086.9995652
GCAGTGG1552950.074.023373
CAGTGGT1659900.069.2130054
AGTGGTA1693700.067.900475
TGGTATC1734300.066.1106957
GTGGTAT1752100.065.586626
GGTATCA1751850.065.4430168
GTATCAA1786200.064.127379
CAACGCA1785000.036.1284712-13
ATCAACG1798900.035.91792710-11
GCAGAGT1804400.035.59866716-17
AGAGTAC1800150.035.5476118-19
ACGCAGA1829750.035.22102714-15
TACATGG1819850.034.56411422-23
AGTACAT1892450.033.7275820-21
CATGGGG532750.033.2096424-25
TCAACGC1774350.032.24654412-13
CATGGGA1206700.031.63370324-25
CAGAGTA1811150.031.48110418-19
Produced by FastQC (version 0.11.5)