FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n02_dpErk_WE_514hpf_H1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n02_dpErk_WE_514hpf_H1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11410106
Sequences flagged as poor quality0
Sequence length110
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC344960.30232847968283555No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA225980.19805249837293362No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT213560.1871674110652434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1158900.096.932531
AGCAGTG1291800.086.852412
GCAGTGG1512850.074.1488343
CAGTGGT1614300.069.470874
AGTGGTA1638500.068.539555
TGGTATC1681700.066.567577
GTGGTAT1698050.066.100016
GGTATCA1697700.065.9438558
GTATCAA1733050.064.590619
CAACGCA1732500.036.37921512-13
ATCAACG1740350.036.27188510-11
AGAGTAC1746250.035.8270918-19
GCAGAGT1751950.035.7738816-17
ACGCAGA1776150.035.4458814-15
TACATGG1767550.034.763322-23
AGTACAT1837250.033.93724420-21
CATGGGG508500.033.64295624-25
TCAACGC1719000.032.51120412-13
CATGGGA1180900.031.86916224-25
CAGAGTA1755800.031.66617618-19
Produced by FastQC (version 0.11.5)