FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n02_dpErk_WE_514hpf_G1_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n02_dpErk_WE_514hpf_G1_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11562152
Sequences flagged as poor quality0
Sequence length110
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC349470.3022534213354054No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA232150.2007844214468033No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT217030.18770727110316487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1178500.097.1091841
AGCAGTG1317800.086.7259452
GCAGTGG1549900.073.713513
CAGTGGT1649150.069.136264
AGTGGTA1680300.067.966135
TGGTATC1719300.066.2681357
GTGGTAT1734250.065.839666
GGTATCA1737600.065.529738
GTATCAA1772850.064.2299969
CAACGCA1767350.036.38706212-13
ATCAACG1782250.036.14556510-11
AGAGTAC1781450.035.8193618-19
GCAGAGT1789300.035.7999616-17
ACGCAGA1812950.035.46036514-15
TACATGG1803350.034.7816522-23
AGTACAT1872050.033.9622920-21
CATGGGG528600.033.6521724-25
TCAACGC1760350.032.3190512-13
CATGGGA1193450.032.1263424-25
AACGCAG1798350.031.64625514-15
Produced by FastQC (version 0.11.5)