FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n02_dpErk_WE_514hpf_G1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n02_dpErk_WE_514hpf_G1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11220335
Sequences flagged as poor quality0
Sequence length110
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC344260.30681793368914567No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA217300.1936662318905808No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT209930.18709780055586575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1133300.096.946241
AGCAGTG1265050.086.716772
GCAGTGG1488400.073.683293
CAGTGGT1585750.069.0615844
AGTGGTA1613250.067.984965
TGGTATC1661150.065.865647
GTGGTAT1677900.065.311746
GGTATCA1678900.065.226498
GTATCAA1712950.063.9031529
CAACGCA1709600.036.0325112-13
ATCAACG1722500.035.8296310-11
GCAGAGT1728700.035.4496916-17
AGAGTAC1722200.035.44648418-19
ACGCAGA1749650.035.13934714-15
TACATGG1744500.034.40236722-23
AGTACAT1812100.033.55774320-21
CATGGGG514800.033.23269724-25
TCAACGC1698000.032.1622812-13
CATGGGA1151050.031.61942924-25
AACGCAG1739450.031.40634214-15
Produced by FastQC (version 0.11.5)