FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n02_dpErk_WE_514hpf_G1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n02_dpErk_WE_514hpf_G1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10967995
Sequences flagged as poor quality0
Sequence length110
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC332850.3034738801394421No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA217510.19831336538720157No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT207820.18947856923713038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1114650.097.251921
AGCAGTG1244750.086.947232
GCAGTGG1461050.073.9818043
CAGTGGT1558000.069.3329854
AGTGGTA1584350.068.282555
TGGTATC1622150.066.469587
GTGGTAT1639950.065.91066
GGTATCA1638650.065.827778
GTATCAA1672350.064.4888159
CAACGCA1669450.036.3539612-13
ATCAACG1682500.036.17315710-11
GCAGAGT1687600.035.80784616-17
AGAGTAC1684850.035.7679318-19
ACGCAGA1709500.035.48224314-15
TACATGG1706150.034.78900522-23
AGTACAT1769850.033.95175620-21
CATGGGG501400.033.22202324-25
TCAACGC1660300.032.46835712-13
CATGGGA1127100.032.0567624-25
CAGAGTA1693100.031.72803918-19
Produced by FastQC (version 0.11.5)