FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n02_dpErk_WE_514hpf_G1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n02_dpErk_WE_514hpf_G1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10667248
Sequences flagged as poor quality0
Sequence length110
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC323020.3028147465963105No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA213770.200398453284296No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT199960.18745228385053014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1085250.096.615981
AGCAGTG1213000.086.347332
GCAGTGG1420850.073.67393
CAGTGGT1514200.069.095774
AGTGGTA1543850.067.854885
TGGTATC1585350.065.897437
GTGGTAT1600500.065.379346
GGTATCA1600500.065.271028
GTATCAA1633650.063.9028749
CAACGCA1628350.036.35591512-13
ATCAACG1636800.036.19982510-11
AGAGTAC1640350.035.75992218-19
GCAGAGT1646500.035.7442916-17
ACGCAGA1666550.035.47783314-15
TACATGG1663650.034.6450922-23
AGTACAT1722550.033.89821620-21
CATGGGG489550.033.43557724-25
TCAACGC1617800.032.21977612-13
CATGGGA1094900.031.97494524-25
AACGCAG1657650.031.44094514-15
Produced by FastQC (version 0.11.5)