FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n02_dpErk_WE_514hpf_F1_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n02_dpErk_WE_514hpf_F1_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12351654
Sequences flagged as poor quality0
Sequence length110
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC369960.2995226388303947No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA241780.1957470635106845No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT233520.18905970002074218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1258600.096.9040451
AGCAGTG1407550.086.5595552
GCAGTGG1642250.074.1592943
CAGTGGT1749550.069.5592964
AGTGGTA1779350.068.475465
TGGTATC1822100.066.672367
GTGGTAT1837250.066.234996
GGTATCA1837600.066.108238
GTATCAA1874050.064.709989
CAACGCA1871800.036.6055112-13
ATCAACG1884400.036.38571510-11
GCAGAGT1890600.036.095316-17
AGAGTAC1884500.036.09013718-19
ACGCAGA1912950.035.76414-15
TACATGG1907800.035.001822-23
AGTACAT1985600.034.11876320-21
CATGGGG561300.033.52193524-25
TCAACGC1860750.032.5775912-13
CATGGGA1266150.032.10368724-25
CAGAGTA1897550.031.87061318-19
Produced by FastQC (version 0.11.5)