FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n02_dpErk_WE_514hpf_F1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n02_dpErk_WE_514hpf_F1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12488764
Sequences flagged as poor quality0
Sequence length110
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC376550.30151102222766No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA246510.19738542581155347No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT234730.1879529471451298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1261600.096.946121
AGCAGTG1406700.086.805892
GCAGTGG1653750.073.7253653
CAGTGGT1763750.069.0582354
AGTGGTA1796350.067.912175
TGGTATC1844750.065.890877
GTGGTAT1862800.065.408216
GGTATCA1864550.065.208698
GTATCAA1899900.064.003879
CAACGCA1906100.036.03002512-13
ATCAACG1917850.035.83075310-11
GCAGAGT1924550.035.45735516-17
AGAGTAC1919150.035.41107618-19
ACGCAGA1952700.035.1057214-15
TACATGG1941700.034.37936822-23
AGTACAT2019350.033.54588320-21
CATGGGG571900.032.86338424-25
TCAACGC1892400.032.18526512-13
CATGGGA1286600.031.69768324-25
AACGCAG1940000.031.37615214-15
Produced by FastQC (version 0.11.5)