FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n02_dpErk_WE_514hpf_F1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n02_dpErk_WE_514hpf_F1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11879602
Sequences flagged as poor quality0
Sequence length110
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC355250.29904200494258987No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA233450.19651331753370188No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT223890.18846590988485978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1215400.096.943981
AGCAGTG1357750.086.75572
GCAGTGG1588250.074.108413
CAGTGGT1692700.069.41864
AGTGGTA1724700.068.277915
TGGTATC1770000.066.291657
GTGGTAT1786350.065.8451466
GGTATCA1789250.065.5795448
GTATCAA1823300.064.266719
CAACGCA1818050.036.3194112-13
ATCAACG1827750.036.1866110-11
GCAGAGT1836800.035.7101716-17
AGAGTAC1835000.035.6594318-19
ACGCAGA1864450.035.37957414-15
TACATGG1857950.034.61242722-23
AGTACAT1927700.033.7976720-21
CATGGGG541550.033.19790324-25
TCAACGC1806650.032.42941712-13
CATGGGA1231600.031.88698224-25
AACGCAG1854950.031.6133114-15
Produced by FastQC (version 0.11.5)