FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n02_dpErk_WE_514hpf_F1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n02_dpErk_WE_514hpf_F1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12446909
Sequences flagged as poor quality0
Sequence length110
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC380080.305360953470456No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA241470.19399997220193385No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT233800.1878377997300374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1257800.097.0148851
AGCAGTG1408600.086.511522
GCAGTGG1649200.073.766713
CAGTGGT1750250.069.4495244
AGTGGTA1784100.068.248255
TGGTATC1829500.066.3928157
GTGGTAT1847450.065.880476
GGTATCA1848650.065.742958
GTATCAA1887450.064.332169
CAACGCA1887200.036.3115912-13
ATCAACG1896750.036.18698510-11
GCAGAGT1909850.035.75597816-17
AGAGTAC1908550.035.68654318-19
ACGCAGA1933600.035.41602714-15
TACATGG1925000.034.74172222-23
CATGGGG569500.033.81110424-25
AGTACAT2009000.033.79512420-21
TCAACGC1873000.032.40397612-13
CATGGGA1271150.031.79552724-25
AACGCAG1918200.031.63499814-15
Produced by FastQC (version 0.11.5)